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Stellaはマウス始原生殖細胞のCGメチル化リプログラミングに資する
*歐陽 允健[1], 鳥山 敬祐[1], 井上 梓[2], 中村 肇伸[3], 仲野 徹[4], Yi Zhang[5], 佐々木 裕之[1]
[1]九州大・生医研・エピゲノム [2]理研・IMS・疾患エピゲノム遺伝 [3]長浜バイオ大・エピジェネティック制御 [4]大阪大・病因解析 [5]Dept. Genet, Harvard Med. School

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Mouse primordial germ cells (PGCs) undergo passive and active genome-wide demethylation. Stella, a small protein in PGCs and oocytes, interferes with the recruitment of CG methylation maintenance factor Uhrf1 to chromatin in cell lines. Local bisulfite sequencing in PGCs suggests that Stella affects CG methylation.
We here report the genome-wide CG hypermethylation in Stella KO PGCs, especially intergenic retrotransposons. Furthermore, Stella works downstream of Prdm14 (the master regulator of epigenetic reprogramming), but independently of Tet1, in PGCs. Our study reveals a new role for Stella in epigenetic reprogramming of PGC.
keywords: DNAメチル化；エピジェネティックリプログラミング；始原生殖細胞

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Stella contributes to CG methylation reprogramming in mouse primordial germ cells
*Wan Kin Au Yeung [1], Keisuke Toriyama [1], Azusa Inoue [2], Toshinobu Nakamura [3], Toru Nakano [4], Yi Zhang [5], Hiroyuki Sasaki [1]
[1] Div. Epigenom. Dev, MIB, Kyushu U. [2] Epigenom. Inherit. Lab, IMS, RIKEN [3] Lab. Epigenet. Regul, Nagahama Ins. Bio-Sci. Tech. [4] Dept. Patho, Osaka U. [5] Dept. Genet, Harvard Med. School