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ショウジョウバエRNAメチル化酵素METTL1はtRNAのm⁷G修飾を介して精子形成・稔性を制御する
*金子隼也[1][2], 三好啓太[1][2], 近藤周[1,3], 豊田敦[4], 寺内真[5], 野口英樹[5], 齋藤都暁[1][2]
[1]遺伝研・無脊椎, [2]総研大・遺伝学, [3]東工大・先進工・生命システム, [4]遺伝研・比較ゲノム, [5]データサイエンス共同利用基盤（ROIS-DS）

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RNA methyltransferase-like 1 (METTL1) is known to induce m7G modification into RNA in eukaryote. To explorer the developmental role of m7G, we generated Drosophila METTL1-KO strain and found the mutant shows male fertility defect. In METTL1-KO, mitotic divisions of germline cells are impaired and no elongated spermatid are observed. Biochemical analyses show METTL1 induces m7G into tRNAs in vitro and in vivo, and regulates expression level of m7G target tRNAs, suggesting that the regulation of tRNA stability by METTL1 links to Drosophila fertility via m7G modification.
keywords: m7G RNA修飾, ショウジョウバエ, 稔性

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Drosophila METTL1 regulates spermatogenesis and preserves complete fertility via tRNA m⁷G modification
*Shunya Kaneko [1,2], Keita Miyoshi [1,2], Shu Kondo [1,3], Atsushi Toyoda [4], Makoto Terauchi [5], Hideki Noguchi [5], Kuniaki Saito [1,2]
[1] Invertebrate Genet. Lab., NIG, [2] Dept. Genet., SOKENDAI, [3] Dept. of Biol. Sci. Tech., Tokyo Univ. of Sci., [4] Comparative Genomics, NIG, [5] Center for Genome Info., ROIS-DS